Kalirin-7 plays the neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation

Kalirin-7 plays the neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation

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Objective(s): The study explored the neuroprotective role of Kalirin-7 (Kal-7) in Neuro-2A cells after oxygen-glucose deprivation and reperfusion (OGD/R) treatment.Materials and Methods: The study used an OGD/R model of mouse Neuro-2A neuroblastoma cells in vitro.Cells were transfected with pCAGGS-Kal-7 to up-regulating Breathe Easy kal-7.

Then cell proliferation and apoptosis were respectively analyzed by Trypan blue exclusion method and flow cytometry.To examine the involvement of Rac1, cells were treated with Rac1-GTP inhibitor NSC23766 before treatment with OGD/R.Expressions of Bax, Bcl-2, Rac1, and down-stream targets of Rac1 were analyzed by Western blot.

Results: Kal-7 significantly decreased OGD/R induced FLAVEL Milano 100 MLN10CRK Electric Range Cooker - Black cell apoptosis (P.